引物名称 | 序列(5'→3') |
CBF1引物1 | CCGGAATTCCGTTAGGACTTCTT |
CBF1引物2 | TCCTCGCCCTTGCTCACCATT |
EGFP引物1 | ATGGTGAGCAAGGGCGAGGA |
EGFP引物2 | ACGCGTCGACTTACTTGTACAG |
(1)在PCR扩增启动子CBF1时,CBF1引物1与模板链结合后,在
(2)限制酶及识别序列如表2所示。构建CBF1-EGFP重组型启动子质粒时,要选择
表2
限制酶 | BamHI | EcoRI | SalI | SmaI |
识别和切割序列 | G↓GATCC | G↓AATTC | G↓TCGAC | GGG↓CCC |
(3)检测含CBF1-EGFP重组型启动子质粒是否构建成功时,将重组质粒导入酵母菌。培养后将酵母菌涂布到含有
(4)EGFP基因能表达荧光蛋白,蛋白质含量与荧光强度呈正相关。为比较POL1~POL5、CBF1这6种启动子的作用强度,应分别检测
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同类型试题
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y = sin x, x∈R, y∈[–1,1],周期为2π,函数图像以 x = (π/2) + kπ 为对称轴
y = arcsin x, x∈[–1,1], y∈[–π/2,π/2]
sin x = 0 ←→ arcsin x = 0
sin x = 1/2 ←→ arcsin x = π/6
sin x = √2/2 ←→ arcsin x = π/4
sin x = 1 ←→ arcsin x = π/2
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y = sin x, x∈R, y∈[–1,1],周期为2π,函数图像以 x = (π/2) + kπ 为对称轴
y = arcsin x, x∈[–1,1], y∈[–π/2,π/2]
sin x = 0 ←→ arcsin x = 0
sin x = 1/2 ←→ arcsin x = π/6
sin x = √2/2 ←→ arcsin x = π/4
sin x = 1 ←→ arcsin x = π/2
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